Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
Usage Physical State: Freeze-dried solidStorage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Purity:The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Stabilizer:15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) Preservative:None(Warning:Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.) Suggested Working Concentration or Dilution Range:1:500 - 1:5,000 for immunohisto/cytochemistry 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates 1:10,000 - 1:200,000 for Western blotting with ECL substratesDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
ConjugateHorseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.
Images & ReferencesThis product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.
AngioBio除了血管生成和病理研究领域外,我们最近还扩展到植物和农业研究场所。我们拥有最多的植物研究抗体,包括2000多种针对拟南芥,水稻和玉米蛋白的抗体。许多新抗体将很快添加到我们的网站。 如果您的目标蛋白不在清单中,请给我们发送电子邮件以将其添加到“ 愿望清单”中。如果有抗体,我们将与您联系。
当前可用的拟南芥抗体:
MSU_locus# | 蛋白质名称 |
AT4G34000 | ABF3脱落酸不敏感的5样蛋白6 |
AT3G24650 | 包含ABI3 B3域的转录因子ABI3 |
AT2G40220 | ABI4乙烯反应转录因子ABI4 |
AT2G36270 | ABI5蛋白脱落酸不敏感5 |
AT4G02980 | ABP1生长素结合蛋白1 |
AT4G33430 | BAK1 BRASSINOSTEROID敏感性1相关受体激酶1 |
AT2G39660 | BIK1丝氨酸/苏氨酸蛋白激酶BIK1 |
AT5G28540 | BIP1发光结合蛋白1 |
AT4G00450 | CCT RNA聚合酶II转录介体 |
AT2G03670 | CDC48B细胞分裂控制蛋白48-B |
AT5G25110 | CIPK25 CBL相互作用的丝氨酸/苏氨酸蛋白激酶25 |
AT2G32950 | COP1 E3泛素蛋白连接酶COP1 |
AT3G57530 | CPK32钙依赖性蛋白激酶32 |
AT1G26830 | CUL3 cullin 3 |
AT1G26770 | EXPA10膨胀蛋白A10 |
AT4G25630 | FIB2 rRNA 2'-O-甲基转移酶原纤维蛋白2 |
AT5G46330 | FLS2 LRR受体样丝氨酸/苏氨酸蛋白激酶FLS2 |
AT1G55325 | GCT RNA聚合酶II转录介体 |
AT5G20630 | GER3种蛋白样蛋白亚家族3成员3 |
AT5G49720 | GH9A1内切葡聚糖酶25 |
AT2G26150 | HSFA2热应激转录因子A-2 |
AT4G19690 | IRT1 Fe(2+)转运蛋白1 |
AT5G13220 | JAZ10蛋白TIFY 9 |
AT3G17860 | JAZ3蛋白TIFY 6B |
AT2G26650 | KT1钾通道AKT1 |
AT5G49880 | MAD1有丝分裂纺锤体装配检查点蛋白MAD1 |
AT5G41910 | MED10A介体复合体,Med10亚基 |
AT1G26665 | MED10b介体复合物,Med10亚基 |
AT3G01435 | Med11假设蛋白 |
AT1G15780 | MED15假设蛋白 |
AT2G22370 | MED18假设蛋白 |
AT5G12230 | MED19A假设蛋白 |
AT5G19480 | MED19b假设蛋白 |
AT3G28230 | MED20a假设蛋白 |
AT4G04780 | MED21蛋白介质21 |
AT1G07950 | 介体复合体的MED22B Surfeit基因座蛋白5亚基 |
AT1G23230 | MED23假设蛋白 |
AT3G09180 | MED27假设蛋白 |
AT3G52860 | MED28假设蛋白 |
AT5G19910 | MED31 RNA聚合酶II转录亚基的介体31 |
AT1G11760 | MED32假设蛋白 |
AT5G02850 | MED4富含羟脯氨酸的糖蛋白家族蛋白 |
AT3G21350 | MED6 RNA聚合酶转录调控介质样蛋白 |
AT5G03500 | MED7b介体复合物,Med7亚基 |
AT2G03070 | MED8介体亚基8 |
AT1G55080 | MED9假设蛋白 |
AT1G32320 | MKK10 MAP激酶激酶10 |
AT5G40440 | MKK3丝裂原激活的蛋白激酶激酶3 |
AT2G43790 | MPK6丝裂原激活的蛋白激酶6 |
AT1G32640 | MYC2转录因子MYC2 |
AT4G19170 | NCED4假定的类胡萝卜素裂解双加氧酶4 |
AT1G64280 | NPR1调节蛋白NPR1 |
AT4G04910 | NSF囊泡融合酶 |
AT2G16430 | PAP10紫色酸性磷酸酶10 |
AT1G25540 | PFT1植物色素和开花时间调节蛋白1 |
AT2G33770 | PHO2假定的泛素结合酶E2 24 |
AT2G43010 | PIF4转录因子PIF4 |
AT2G46970 | PIL1转录因子PIL1 |
AT3G59060 | PIL6转录因子PIF5 |
AT1G73590 | PIN1生长素外排载体成分1 |
AT5G57090 | PIN1生长素外排载体成分2 |
AT1G70940 | PIN1生长素外排载体成分3 |
AT2G01420 | PIN1生长素外排载体组分4 |
AT5G16530 | PIN5推定的生长素外排载体成分8 |
AT1G23080 | PIN7生长素外排载体组分7 |
AT3G20840 | PLT1类AP2的乙烯反应性转录因子PLT1 |
AT1G51190 | PLT2类AP2的乙烯反应性转录因子PLT2 |
AT1G11580 | PMEPCRA双功能果胶酯酶18 / rRNA N-糖基化酶 |
AT1G44910 | PRP40A mRNA加工前蛋白40A |
AT2G43490 | RAB GTPase激活蛋白 |
AT3G59570 | 包含RabGAP / TBC域的蛋白 |
AT1G31360 | RECQL2 ATP依赖性DNA解旋酶Q样2 |
AT2G48110 | REF4减少了表皮荧光4蛋白 |
AT3G23590 | RFR1 REF4相关的1蛋白 |
AT5G20170 | RNA聚合酶II转录介体 |
AT1G20090 | ROP2 Rac样GTP结合蛋白ARAC4 |
AT3G54220 | SCR蛋白稻草人 |
AT4G04920 | SFR6对冷冻6蛋白敏感 |
AT4G37650 | SHR蛋白短根 |
AT4G16340 | SPK1鸟嘌呤核苷酸交换因子/ GTPase结合/ GTP结合蛋白 |
at4g02430 | 剪接因子SR1B |
AT1G09960 | SUT4蔗糖转运蛋白4 |
AT3G04740 | SWP RNA聚合酶II转录介体 |
AT3G03800 | 1B / 2/3中的SYP131语法 |
AT4G09070 | 介导复合体20亚基的TATA结合相关因子(TRF) |
AT1G70700 | TIFY7蛋白TIFY 7 |
AT3G11260 | WOX5 WUSCHEL相关的homeobox 5 |
AT5G46350 | WRKY8推定的WRKY转录因子8 |
AT2G17950 | WUS蛋白 |
AT1G10550 | XTH33木葡聚糖:木葡糖基转移酶 |